new general method for separation of nucleic acids

Agarose gel chromatography at 2.5 M NaCl was applied to separation of nucleic acid mixtures extracted from bacteria, plants and vertebrates. In all cases 1t was possible to obtain, in a single chromatographic run, a DNA fraction containing less than 1 wt. % of alkali-labile polynucleotides, a low-mo...

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Veröffentlicht in:	Preparative biochemistry 1974, Vol.4 (6), p.509-522
Hauptverfasser:	Petrovic, S.L, Petrovic, J.S, Markovic, R.A, Knezevic, Z.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Carbon Radioisotopes Cell Nucleus - analysis Chick Embryo Chromatography, Gel DNA - isolation & purification Liver - analysis Methods Molecular Weight Phosphorus Radioisotopes plant biochemistry plant physiology Rats Reticulocytes - analysis RNA - isolation & purification RNA, Bacterial - isolation & purification Salmonella typhimurium - analysis
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Zusammenfassung:	Agarose gel chromatography at 2.5 M NaCl was applied to separation of nucleic acid mixtures extracted from bacteria, plants and vertebrates. In all cases 1t was possible to obtain, in a single chromatographic run, a DNA fraction containing less than 1 wt. % of alkali-labile polynucleotides, a low-molecular weight RNA fraction consisting mainly of tRNA, and a high-molecular weight RNA fraction comprising polyribonucleotides with more than approximately 100 nucleotidyl residues per chain.
ISSN:	0032-7484 2331-0510
DOI:	10.1080/00327487408061552