Application of Thiamine as A Fluorogenic Substrate in the Determination of Hydrogen Peroxide Based on the Catalytic Effect of Hemin

A rapid and sensitive fluorimetric method has been developed for the determination of trace hydrogen peroxide (0-5.0 × 10 −6 mol/l) and, indirectly, glucose (3.0 × 10 −7 - 3.5 × 10 −5 mol/l), based on the catalytic effect of hemin on the thiamine, a new fluorogenic substrate, and hydrogen peroxide system at pH=8.0. Using the fixed time method, the detection limits of hydrogen peroxide and glucose are 3.0 × 10 −8 mol/l and 3.0 × 10 −7 mol/l, respectively. This method is simple, inexpensive and has been applied successfully to the determination of glucose in human sera with relative standard deviation of 2.4-3.1%. *The State Laboratory for Tumor Cell Engineering. Department of Biology, Xiamen University. Xiamen 361005, P.R. China.