Determination of Glutamine in Mammalian Cell Cultures with a Flow Injection Analysis/Wall-Jet Electrode System

A flow injection analysis/wall-jet electrode system coupled to a reactor packed with controlled pore glass beads containing coimmobilized glutaminase /glutamate oxidase was investigated for glutamine determination in mammalian cell cultures. In the reactor, glutamine was converted to hydrogen peroxi...

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Veröffentlicht in:	Analytical letters 1995-03, Vol.28 (4), p.593-603
Hauptverfasser:	Huang, Yu Liang, Khoo, Soo Beng, Yap, Miranda G. S.
Format:	Artikel
Sprache:	eng
Schlagworte:	amperometric biosensor Biological and medical sciences Biosensors Biotechnology cell cultures flow injection analysis Fundamental and applied biological sciences. Psychology Glutamine Methods. Procedures. Technologies Various methods and equipments wall-jet electrode
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Zusammenfassung:	A flow injection analysis/wall-jet electrode system coupled to a reactor packed with controlled pore glass beads containing coimmobilized glutaminase /glutamate oxidase was investigated for glutamine determination in mammalian cell cultures. In the reactor, glutamine was converted to hydrogen peroxide which was detected amperometrically at the wall-jet electrode controlled at +0.65 V (vs Ag/AgCl, saturated KCl). The system was tested for its stability, linearity and precision of response to glutamine. Finally, it was applied to determination of glutamine in various mammalian cell cultures.
ISSN:	0003-2719 1532-236X
DOI:	10.1080/00032719508001120