Development and Validation of Murabutide Assay by High Performance Capillary Electrophoresis

The objective of this study was to develop an analytical technique for the assay of murabutide, a synthetic peptide, by high performance capillary electrophoresis. The analyte concentration was monitored by UV detection. Murabutide was eluted by using pH 8.0 borate buffer as the running buffer. The effect of the running buffer concentration on peak sharpness was investigated. Increasing concentration of the running buffer increased peak migration time and also improved the peak sharpness significantly. The assay technique was validated by plotting the standard curve and performing the inter-and intra-day variability studies.