Spegtrophotometrig Determination of α -Tocopherol Acetate in Soft Capsules

Two spectrophotometric methods have been described for the determination of α -tocopherol acetate (vitamin E) in soft capsules. The first method applies the orthogonal function method under least squares. The quadratic coefficients calculated over the wavelength range 277 - 304 nm at 4-nm intervals in chloroform were reproducible and independent of ethyl oleate concentration. The second method applies the second derivative (D 2 ) to correct for irrelevant absorbance due to ethyl oleate The peak trough amplitude at 271 - 287 nm was in linear correlation to the concentration of vitamin E. The recoveries obtained using the proposed methods agreed with those obtained using the official proceduce.