HPLC Assay for Trimethoprim in Plasma and Urine

HPLC Assay for Trimethoprim in Plasma and Urine

A sensitive HPLC method for the quantitation of trimethoprim in plasma and urine was developed using fluorescence detection. Plasma (or urine) samples were made basic by the addition of 3.8N sodium hydroxide and extracted with chloroform:2-propanol (95:5). After evaporation of the organic layer, a p...

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Veröffentlicht in:Analytical letters 1978-01, Vol.11 (11), p.967-973
Hauptverfasser: Gautam, Sushen R., Chungi, Vinod S., Bourne, David W. A., Munson, James W.
Format: Artikel
Sprache:eng
Schlagworte:
fluorescence detection
HPLC
plasma and urine levels
trimethoprim
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Zusammenfassung:A sensitive HPLC method for the quantitation of trimethoprim in plasma and urine was developed using fluorescence detection. Plasma (or urine) samples were made basic by the addition of 3.8N sodium hydroxide and extracted with chloroform:2-propanol (95:5). After evaporation of the organic layer, a portion of the residue was analyzed by HPLC with fluorescence detection. The minimum detectable quantity is 0.1 μg/ml for this method. This method has been applied to the analysis of plasma and urine obtained from subjects after a single 160 mg dose of trimethoprim.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032717808059748