Up-regulation of MUC5AC mRNA Expression in Endotoxin-induced Otitis Media

Up-regulation of MUC5AC mRNA Expression in Endotoxin-induced Otitis Media

We investigated the expression levels of MUC5AC in endotoxin-induced otitis media with effusion (OME) in the rat using competitive polymerase chain reaction (PCR) and the morphology of middle ear mucosa using transmission electron microscopy (TEM). Experimental OME in the rat was induced after middl...

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Veröffentlicht in:Acta oto-laryngologica 2001-04, Vol.121 (3), p.364-370
1. Verfasser: Young Tae Kim, Hak Hyun Jung, Tae Ok Ko, Seo Jin Kim
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology
Ear, Middle - pathology
Endotoxin Mucin Otitis Media With Effusion Rats Ultrastructure
Ent. Stomatology
Escherichia coli
Investigative techniques, diagnostic techniques (general aspects)
Lipopolysaccharides - pharmacology
Medical sciences
Microscopy, Electron
Mucin 5AC
Mucins - genetics
Non tumoral diseases
Otitis Media with Effusion - genetics
Otitis Media with Effusion - pathology
Otorhinolaryngology. Stomatology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Polymerase Chain Reaction
Rats
Rats, Sprague-Dawley
RNA, Messenger - genetics
Up-Regulation - genetics
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Zusammenfassung:We investigated the expression levels of MUC5AC in endotoxin-induced otitis media with effusion (OME) in the rat using competitive polymerase chain reaction (PCR) and the morphology of middle ear mucosa using transmission electron microscopy (TEM). Experimental OME in the rat was induced after middle ear instillation of Escherichia coli lipopolysaccharides (LPS). Middle ear mucosa were obtained at 0 h, 12 h, Day 1, Day 3, Day 7 and Day 14 and reverse transcriptase (RT)-PCRs were then performed for the identification of MUC1, MUC2, MUC5AC and submandibular mucin 1 expression, followed by competitive PCRs for MUC5AC
ISSN:0001-6489
1651-2251
DOI:10.1080/000164801300102798