Initial Characterization of the Enzyme and Cloning of Genes Involved in the Enantioselective Epoxyalkane Degradation by Xanthobacter PY2

Initial Characterization of the Enzyme and Cloning of Genes Involved in the Enantioselective Epoxyalkane Degradation by Xanthobacter PY2

The epoxyalkane-degrading enzyme of Xanthobacter Py2 was studied in crude extracts. NAD and a low molecular weight fraction which can be replaced by DTT are necessary for the conversion of epoxyalkanes into ketones. The genes responsible for the epoxyalkane-degrading enzyme were cloned and expressed...

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Veröffentlicht in:Biocatalysis 1994, Vol.10 (1-4), p.227-232
Hauptverfasser: Swaving, J., Weijers, C. A. G. M., Chion, C. K. Chan Kwo, Leak, D. J., Ooyen, A. J. J. Van, Bont, J. A. M. De
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Sprache:eng
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Zusammenfassung:The epoxyalkane-degrading enzyme of Xanthobacter Py2 was studied in crude extracts. NAD and a low molecular weight fraction which can be replaced by DTT are necessary for the conversion of epoxyalkanes into ketones. The genes responsible for the epoxyalkane-degrading enzyme were cloned and expressed in Xanthobacter autotrophics GJ10.
ISSN:1024-2422
0886-4454
1029-2446
DOI:10.3109/10242429409065232