Renal differentiation from adult spermatogonial stem cells

Renal differentiation from adult spermatogonial stem cells

Abstract There is considerable interest in the use of multi-potent stem cells in kidney tissue regeneration. We studied if spermatogonial stem cells have the ability to undergo kidney differentiation. Spermatogonial stem cell differentiation was induced using in vitro and ex vivo co-culture techniqu...

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Veröffentlicht in:Renal failure 2013-11, Vol.35 (10), p.1387-1391
Hauptverfasser: Heer, Rakesh, Hepburn, Anastasia C., Williamson, Stuart C., Kennedy, Amy, El-Sherif, Amira, Soomro, Naeem A., Brown, Colin D.A., Robson, Craig N.
Format: Artikel
Sprache:eng
Schlagworte:
Adult Stem Cells - physiology
Animals
Cell Differentiation
Differentiation
Fibroblasts - physiology
Humans
kidney
Kidney - cytology
Kidney - embryology
Kidney - physiology
Mice
Mice, Transgenic
Organ Culture Techniques
Paracrine Communication
Regeneration
spermatogonial stem cells
stem cells
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Zusammenfassung:Abstract There is considerable interest in the use of multi-potent stem cells in kidney tissue regeneration. We studied if spermatogonial stem cells have the ability to undergo kidney differentiation. Spermatogonial stem cell differentiation was induced using in vitro and ex vivo co-culture techniques. Conditioned media from human kidney fibroblasts induced the expression of epithelial and endothelial lineages in spermatogonial stem cells, consistent with nephrogenesis. Furthermore, we showed that these cells up-regulated renal tubular-specific markers alkaline phosphatase, mineralocorticoid receptor, renal epithelial sodium channel and sodium-glucose transporter-2 (p 
ISSN:0886-022X
1525-6049
DOI:10.3109/0886022X.2013.828266