The Activation Function of Steroidogenic Factor-1 is Impaired in Acth-Resistant Y1 Mutants
This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to...
Gespeichert in:
| Veröffentlicht in: | Endocrine research 2000-01, Vol.26 (4), p.1005-1009 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1009 |
|---|---|
| container_issue | 4 |
| container_start_page | 1005 |
| container_title | Endocrine research |
| container_volume | 26 |
| creator | Frigeri, Claudia Schimmer, Bernard P. |
| description | This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to interact with the coactivator, GRIP1 was affected as determined in a modified mammalian 2-hybrid assay. These findings indicate that the mutants harbor a defect affecting the activation function of SF1. |
| doi_str_mv | 10.3109/07435800009048631 |
| format | Article |
| fullrecord | <record><control><sourceid>informahealthcare_cross</sourceid><recordid>TN_cdi_informahealthcare_journals_10_3109_07435800009048631</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_3109_07435800009048631</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-b86512738f10ed84a245220a0eb45f59c247deea5e4ea10a59d0d4cb64d829233</originalsourceid><addsrcrecordid>eNp9kF9LwzAUxYMobk4_gC-SL1C9-dOuRV-GOB1MBJ0P-lLSJLUZXTOSVNm3t3UDEdH7cg_c8ztcDkKnBM4ZgewCxpzFKXSTAU8TRvbQkMSMRpxCso-G_T3qDQN05P0SgDAAdogGhJAs4ZAN0eui0ngig3kXwdgGT9tGfglb4qegnTXKvunGSDwVMlgXEWw8nq3WwjitsGl6uIoetTc-iCbgF4Lv2175Y3RQitrrk90eoefpzeL6Lpo_3M6uJ_NIcqAhKtIkJnTM0pKAVikXlMeUggBd8LiMM0n5WGktYs21ICDiTIHiski4SmlGGRshss2VznrvdJmvnVkJt8kJ5H1P-a-eOuZsy6zbYqXVN7ErpjNcbQ2mKa1biQ_rapUHsamtK51opPF99t_5lz_wSos6VFI4nS9t65quj3---wShxocx</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The Activation Function of Steroidogenic Factor-1 is Impaired in Acth-Resistant Y1 Mutants</title><source>MEDLINE</source><source>Taylor & Francis</source><source>Taylor & Francis Medical Library - CRKN</source><creator>Frigeri, Claudia ; Schimmer, Bernard P.</creator><creatorcontrib>Frigeri, Claudia ; Schimmer, Bernard P.</creatorcontrib><description>This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to interact with the coactivator, GRIP1 was affected as determined in a modified mammalian 2-hybrid assay. These findings indicate that the mutants harbor a defect affecting the activation function of SF1.</description><identifier>ISSN: 0743-5800</identifier><identifier>EISSN: 1532-4206</identifier><identifier>DOI: 10.3109/07435800009048631</identifier><identifier>PMID: 11196409</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Adrenal Cortex Neoplasms - genetics ; Adrenal Cortex Neoplasms - pathology ; Adrenocorticotropic Hormone - pharmacology ; Animals ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - physiology ; Drug Resistance ; Drug Synergism ; Fushi Tarazu Transcription Factors ; Homeodomain Proteins ; Mice ; Mutation - physiology ; Nuclear Receptor Coactivator 2 ; Receptors, Cytoplasmic and Nuclear ; Steroidogenic Factor 1 ; Transcription Factors - genetics ; Transcription Factors - physiology ; Tumor Cells, Cultured</subject><ispartof>Endocrine research, 2000-01, Vol.26 (4), p.1005-1009</ispartof><rights>2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-b86512738f10ed84a245220a0eb45f59c247deea5e4ea10a59d0d4cb64d829233</citedby><cites>FETCH-LOGICAL-c402t-b86512738f10ed84a245220a0eb45f59c247deea5e4ea10a59d0d4cb64d829233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/07435800009048631$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/07435800009048631$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,59626,59732,60415,60521,61200,61235,61381,61416</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11196409$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frigeri, Claudia</creatorcontrib><creatorcontrib>Schimmer, Bernard P.</creatorcontrib><title>The Activation Function of Steroidogenic Factor-1 is Impaired in Acth-Resistant Y1 Mutants</title><title>Endocrine research</title><addtitle>Endocr Res</addtitle><description>This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to interact with the coactivator, GRIP1 was affected as determined in a modified mammalian 2-hybrid assay. These findings indicate that the mutants harbor a defect affecting the activation function of SF1.</description><subject>Adrenal Cortex Neoplasms - genetics</subject><subject>Adrenal Cortex Neoplasms - pathology</subject><subject>Adrenocorticotropic Hormone - pharmacology</subject><subject>Animals</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Drug Resistance</subject><subject>Drug Synergism</subject><subject>Fushi Tarazu Transcription Factors</subject><subject>Homeodomain Proteins</subject><subject>Mice</subject><subject>Mutation - physiology</subject><subject>Nuclear Receptor Coactivator 2</subject><subject>Receptors, Cytoplasmic and Nuclear</subject><subject>Steroidogenic Factor 1</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - physiology</subject><subject>Tumor Cells, Cultured</subject><issn>0743-5800</issn><issn>1532-4206</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF9LwzAUxYMobk4_gC-SL1C9-dOuRV-GOB1MBJ0P-lLSJLUZXTOSVNm3t3UDEdH7cg_c8ztcDkKnBM4ZgewCxpzFKXSTAU8TRvbQkMSMRpxCso-G_T3qDQN05P0SgDAAdogGhJAs4ZAN0eui0ngig3kXwdgGT9tGfglb4qegnTXKvunGSDwVMlgXEWw8nq3WwjitsGl6uIoetTc-iCbgF4Lv2175Y3RQitrrk90eoefpzeL6Lpo_3M6uJ_NIcqAhKtIkJnTM0pKAVikXlMeUggBd8LiMM0n5WGktYs21ICDiTIHiski4SmlGGRshss2VznrvdJmvnVkJt8kJ5H1P-a-eOuZsy6zbYqXVN7ErpjNcbQ2mKa1biQ_rapUHsamtK51opPF99t_5lz_wSos6VFI4nS9t65quj3---wShxocx</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Frigeri, Claudia</creator><creator>Schimmer, Bernard P.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20000101</creationdate><title>The Activation Function of Steroidogenic Factor-1 is Impaired in Acth-Resistant Y1 Mutants</title><author>Frigeri, Claudia ; Schimmer, Bernard P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-b86512738f10ed84a245220a0eb45f59c247deea5e4ea10a59d0d4cb64d829233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adrenal Cortex Neoplasms - genetics</topic><topic>Adrenal Cortex Neoplasms - pathology</topic><topic>Adrenocorticotropic Hormone - pharmacology</topic><topic>Animals</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Drug Resistance</topic><topic>Drug Synergism</topic><topic>Fushi Tarazu Transcription Factors</topic><topic>Homeodomain Proteins</topic><topic>Mice</topic><topic>Mutation - physiology</topic><topic>Nuclear Receptor Coactivator 2</topic><topic>Receptors, Cytoplasmic and Nuclear</topic><topic>Steroidogenic Factor 1</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - physiology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frigeri, Claudia</creatorcontrib><creatorcontrib>Schimmer, Bernard P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Endocrine research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frigeri, Claudia</au><au>Schimmer, Bernard P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Activation Function of Steroidogenic Factor-1 is Impaired in Acth-Resistant Y1 Mutants</atitle><jtitle>Endocrine research</jtitle><addtitle>Endocr Res</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>26</volume><issue>4</issue><spage>1005</spage><epage>1009</epage><pages>1005-1009</pages><issn>0743-5800</issn><eissn>1532-4206</eissn><abstract>This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to interact with the coactivator, GRIP1 was affected as determined in a modified mammalian 2-hybrid assay. These findings indicate that the mutants harbor a defect affecting the activation function of SF1.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>11196409</pmid><doi>10.3109/07435800009048631</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0743-5800 |
| ispartof | Endocrine research, 2000-01, Vol.26 (4), p.1005-1009 |
| issn | 0743-5800 1532-4206 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_3109_07435800009048631 |
| source | MEDLINE; Taylor & Francis; Taylor & Francis Medical Library - CRKN |
| subjects | Adrenal Cortex Neoplasms - genetics Adrenal Cortex Neoplasms - pathology Adrenocorticotropic Hormone - pharmacology Animals DNA-Binding Proteins - genetics DNA-Binding Proteins - physiology Drug Resistance Drug Synergism Fushi Tarazu Transcription Factors Homeodomain Proteins Mice Mutation - physiology Nuclear Receptor Coactivator 2 Receptors, Cytoplasmic and Nuclear Steroidogenic Factor 1 Transcription Factors - genetics Transcription Factors - physiology Tumor Cells, Cultured |
| title | The Activation Function of Steroidogenic Factor-1 is Impaired in Acth-Resistant Y1 Mutants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T04%3A08%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-informahealthcare_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Activation%20Function%20of%20Steroidogenic%20Factor-1%20is%20Impaired%20in%20Acth-Resistant%20Y1%20Mutants&rft.jtitle=Endocrine%20research&rft.au=Frigeri,%20Claudia&rft.date=2000-01-01&rft.volume=26&rft.issue=4&rft.spage=1005&rft.epage=1009&rft.pages=1005-1009&rft.issn=0743-5800&rft.eissn=1532-4206&rft_id=info:doi/10.3109/07435800009048631&rft_dat=%3Cinformahealthcare_cross%3E10_3109_07435800009048631%3C/informahealthcare_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/11196409&rfr_iscdi=true |