The Activation Function of Steroidogenic Factor-1 is Impaired in Acth-Resistant Y1 Mutants

This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to...

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Veröffentlicht in:	Endocrine research 2000-01, Vol.26 (4), p.1005-1009
Hauptverfasser:	Frigeri, Claudia, Schimmer, Bernard P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenal Cortex Neoplasms - genetics Adrenal Cortex Neoplasms - pathology Adrenocorticotropic Hormone - pharmacology Animals DNA-Binding Proteins - genetics DNA-Binding Proteins - physiology Drug Resistance Drug Synergism Fushi Tarazu Transcription Factors Homeodomain Proteins Mice Mutation - physiology Nuclear Receptor Coactivator 2 Receptors, Cytoplasmic and Nuclear Steroidogenic Factor 1 Transcription Factors - genetics Transcription Factors - physiology Tumor Cells, Cultured
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Zusammenfassung:	This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to interact with the coactivator, GRIP1 was affected as determined in a modified mammalian 2-hybrid assay. These findings indicate that the mutants harbor a defect affecting the activation function of SF1.
ISSN:	0743-5800 1532-4206
DOI:	10.3109/07435800009048631