Repair Replication and Unscheduled DNA Synthesis in Synchronous Mammalian Cells

Summary Mammalian cell populations, synchronized by the excess thymidine double block technique, were irradiated in the G2 phase of the mitotic cycle with u.v. or 60Co radiations then pulsed with 3H 5-bromo-2-deoxyuridine. Repair replication was demonstrated in each of the three cell lines investiga...

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Veröffentlicht in:	Int. J. Radiat. Biol. 21: No. 2, 127-36 (Feb 1972) 127-36 (Feb 1972), 1972-01, Vol.21 (2), p.127-136
Hauptverfasser:	Richold, Margaret, Arlett, C.F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Autoradiography BIOLOGICAL REPAIR BIOSYNTHESIS Bromodeoxyuridine - pharmacology BUDR Cell Line - metabolism Cell Line - radiation effects CELLS, CULTURED/radiation effects on unscheduled DNA synthesis in hamster and HeLa, repair of gamma and uv Centrifugation, Density Gradient COBALT 60 Cricetinae CRICETULUS DNA - biosynthesis DNA Repair DNA REPLICATION DNA, Neoplasm - biosynthesis DOSE RATES DOSE-RESPONSE RELATIONSHIPS GAMMA RADIATION GAMMA RADIATION/effects on unscheduled DNA synthesis in synchronized hamster and HeLa cells, repair replication of HELA CELLS HeLa Cells - metabolism Humans In Vitro Techniques Lung - cytology MITOSIS N48200 -Life Sciences-Radiation Effects on Cells NUCLEIC ACID, DEOXYRIBO-/radiation effects on unscheduled synthesis of, in synchronized hamster and HeLa cells, repair replication of gamma and uv Radiation Effects RESPONSE MODIFYING FACTORS SYNCHRONOUS CULTURES THYMIDINE Thymidine - metabolism Tritium TRITIUM COMPOUNDS ULTRAVIOLET RADIATION ULTRAVIOLET RADIATION/ effects on unscheduled DNA synthesis in synchronized hamster and HeLa cells, repair replication of Ultraviolet Rays
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Zusammenfassung:	Summary Mammalian cell populations, synchronized by the excess thymidine double block technique, were irradiated in the G2 phase of the mitotic cycle with u.v. or 60Co radiations then pulsed with 3H 5-bromo-2-deoxyuridine. Repair replication was demonstrated in each of the three cell lines investigated after u.v.-irradiation; but no evidence for repair was detected after 60Co-irradiation. The extent of repair did not increase with dose. A correlation between repair replication and unscheduled DNA synthesis was observed.
ISSN:	0955-3002 0020-7616 1362-3095
DOI:	10.1080/09553007214550151