Modulation of Cell-Mediated Immune Response in B16F-10 Melanoma-Induced Metastatic Tumor-Bearing C57BL 6 Mice by Sulforaphane

Effect of sulforaphane on cell-mediated immune response (CMI) was studied in B16F-10 melanoma-induced metastasis-bearing C57BL 6 mice. Administration of sulforaphane significantly enhanced natural killer (NK) cell activity in metastatic tumor-bearing animals (43.17% cell lysis, on day 5) and the act...

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Veröffentlicht in:Immunopharmacology and immunotoxicology 2007-01, Vol.29 (2), p.173-186
Hauptverfasser: Thejass, P., Kuttan, G.
Format: Artikel
Sprache:eng
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Zusammenfassung:Effect of sulforaphane on cell-mediated immune response (CMI) was studied in B16F-10 melanoma-induced metastasis-bearing C57BL 6 mice. Administration of sulforaphane significantly enhanced natural killer (NK) cell activity in metastatic tumor-bearing animals (43.17% cell lysis, on day 5) and the activity was observed earlier than in tumor-bearing control animals (maximum of 9.76% cell lysis, on day 9). Antibody-dependent cellular cytotoxicity also was enhanced significantly in metastatic tumor-bearing animals (41.20% cell lysis on day 9) after sulforaphane administration compared with untreated control tumor-bearing animals (maximum of 12.62% cell lysis on day 15). An early antibody-dependent complement-mediated cytotoxicity also was observed in sulforaphane-treated tumor-bearing animals (26% cell lysis, on day 15). Administration of sulforaphane significantly enhanced the production of IL-2 and IFN-γ in metastatic tumor-bearing animals. In addition, sulforaphane significantly downregulated the serum levels of proinflammatory cytokines such as IL-1β, IL-6, TNF-α, and GM-CSF during metastasis. These data clearly suggest that sulforaphane effectively inhibited the spread of metastatic tumor cells through the stimulation of CMI, upregulation of IL-2 and IFN-γ, and downregulation of proinflammatory cytokines IL-1β, IL-6, TNF-α, and GM-CSF.
ISSN:0892-3973
1532-2513
DOI:10.1080/08923970701511728