Decreased (Ca2++Mg2+)-Stimulated ATPase Activity in Erythrocyte Membranes from Polycythemia Vera Patients

Erythrocytes were hemolyzed in hypotonic phosphate buffer containing 0.5 mmol/1 Ca2+ and the membranes subsequently washed twice in hypotonic tris buffer. The centrifugation was performed in a continuous flow system, which was necessary to obtain maximal ATPase activity. The Mg2+-dependent Ca2+-stim...

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Veröffentlicht in:	Scandinavian journal of clinical and laboratory investigation 1975, Vol.35 (6), p.583-589
Hauptverfasser:	Scharff, O., Foder, B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine triphosphatase Adenosine Triphosphatases - blood Adult Age Factors Aged Calcium - pharmacology calcium affinity Cell Membrane - enzymology Centrifugation Erythrocyte Count erythrocyte membrane Erythrocytes - enzymology Female Humans Kinetics Leukocyte Count Magnesium - pharmacology Male Middle Aged polycythemia Polycythemia Vera - blood Polycythemia Vera - enzymology Stimulation, Chemical
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Zusammenfassung:	Erythrocytes were hemolyzed in hypotonic phosphate buffer containing 0.5 mmol/1 Ca2+ and the membranes subsequently washed twice in hypotonic tris buffer. The centrifugation was performed in a continuous flow system, which was necessary to obtain maximal ATPase activity. The Mg2+-dependent Ca2+-stimulated ATPase activity of 14 patients with polycythemia vera was only 67 per cent (P
ISSN:	0036-5513 1502-7686
DOI:	10.1080/00365517509095784