Single-molecule imaging in solution with total internal reflection excitation

Single-molecule imaging in solution with total internal reflection excitation

Summary form only given.The detection of individual single-chromophore molecules in aqueous solution, first demonstrated with picoliter-sized sample volumes, and soon after with femtoliter-sized volumes, has become routinely achievable. More recently, the imaging of individual single-chromophore mol...

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Bibliographische Detailangaben
Hauptverfasser: Parker, W.C., Davis, L.M., Williams, J.G.K., Middendorf, L.R., Bashford, C.R.
Format: Tagungsbericht
Sprache:eng
Schlagworte:
Bones
Fiber lasers
Laser ablation
Laser excitation
Laser surgery
Optical control
Optical reflection
Optical scattering
Proteins
Surges
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Beschreibung
Zusammenfassung:Summary form only given.The detection of individual single-chromophore molecules in aqueous solution, first demonstrated with picoliter-sized sample volumes, and soon after with femtoliter-sized volumes, has become routinely achievable. More recently, the imaging of individual single-chromophore molecules in very thin layers of aqueous solution, using epifluorescence and total internal reflection (TIR) microscopies has been reported.
DOI:10.1109/CLEO.2000.907311