SERS Biosensor for Label Free Monitoring of Environmental Stress
The increasing threat of environmental contamination with toxins has led to substantial advances in biosensor techniques. So far, there is no label free portable dynamic sensor, which can replace standard ELISA to monitor the levels of toxins intracellular in living cells or organisms. Our group has...
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Zusammenfassung: | The increasing threat of environmental contamination with toxins has led to substantial advances in biosensor techniques. So far, there is no label free portable dynamic sensor, which can replace standard ELISA to monitor the levels of toxins intracellular in living cells or organisms. Our group has developed a silver nanoparticle (AgNPs) based Surface Enhanced Raman Spectroscopy (SERS) sensor to detect levels of stress protein (RAD54) expressed by a human surrogate (yeast) in response to environmental toxins (H 2 O 2 and U.V.). The fabricated SERS sensor allowed label-free and rapid detection over the ELISA and showed good correlation in extracellular detection of RAD54. In our progress towards intracellular detection, we tested three possible techniques (active, passive and peptide facilitated) for efficient delivery of AgNPs. TAT-HA2 facilitated delivery resulted in increased and rapid uptake of AgNPs as compared to passive diffusion. Dynamic monitoring of RAD54 marker expressed by living cells in response to environmental stress using multiplex SERS sensor with targeting and delivery moieties is in progress. |
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DOI: | 10.1109/SBEC.2013.48 |