Length determination of DNA fragments in atomic force microscope images

Length determination of DNA fragments in atomic force microscope images

A processing algorithm was developed to detect and determine the lengths of DNA fragments in atomic force microscope images. The algorithm was designed to account for varying image conditions, fragment sizes, and background contamination, while minimizing the processing time to provide a high throug...

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Bibliographische Detailangaben
Hauptverfasser: Spisz, T.S., D'Costa, N., Seymour, C.K., Hoh, J.H., Reeves, R., Bankman, I.N.
Format: Tagungsbericht
Sprache:eng
Schlagworte:
Atomic force microscopy
Bioinformatics
Couplings
Diseases
DNA
Electrokinetics
Genetics
Genomics
Optical microscopy
Physics
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Beschreibung
Zusammenfassung:A processing algorithm was developed to detect and determine the lengths of DNA fragments in atomic force microscope images. The algorithm was designed to account for varying image conditions, fragment sizes, and background contamination, while minimizing the processing time to provide a high throughput. Although a large enough sample of images has not been tested yet to determine precisely the accuracy, we estimate the accuracy to be within 2 pixels based on analysis of an ideal simulated image and an actual image.
DOI:10.1109/ICIP.1997.632033