Measurement of membrane potentials in arabidopsis thaliana stem epidermal cells

Measurement of membrane potentials in arabidopsis thaliana stem epidermal cells

A method was developed to determine the changes of the Arabidopsis thaliana tissues membrane potentials rapidly. Arabidopsis thaliana stem epidermal cells were labeled by different concentrations of the hydrophobic fluorescence dye DiBAC4(3), and the changes of the membrane potentials were measured...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Shikun Tang, Yongkun Yu, FuKuan Zhao, Qingpeng Sun
Format: Tagungsbericht
Sprache:chi ; eng
Schlagworte:
Arabidopsis thaliana
Biomembranes
Biotechnology
DiBAC4
Epidermis
Fluorescence
Jasmonic acid
Membrane potentials
Microscopy
Sun
Voltage measurement
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Beschreibung
Zusammenfassung:A method was developed to determine the changes of the Arabidopsis thaliana tissues membrane potentials rapidly. Arabidopsis thaliana stem epidermal cells were labeled by different concentrations of the hydrophobic fluorescence dye DiBAC4(3), and the changes of the membrane potentials were measured dynamically by Confocal laser scanning microscope (CLSM). The effects of Jasmonic acid on Arabidopsis thaliana stem epidermal cells membrane potentials were investigated by the established method. The results showed that the best effect is the DiBAC4 (3) final concentration of 5 μmol·L -1 .
DOI:10.1109/RSETE.2011.5965956