Synthesis of Fe3O4/hesperetin compound

The purpose of this work is to modify hesperetin on nanoparticles of Fe 3 O 4 to form a compound of Fe 3 O 4 /hesperetin, where hesperetin is drug and the Fe 3 O 4 is carrier. The particles of Fe 3 O 4 were synthesized by co-precipitation of Fe (III) and Fe (II) with sodium hydroxide. X-ray diffraction (XRD) was used to study the types of the synthesized iron oxide particles. The XRD pattern confirmed that the synthesized particles are mainly Fe 3 O 4 . Scanning electron microscopy shows the particle size of Fe 3 O 4 is about 14 nm on average. The Fe 3 O 4 /hesperetin compound was synthesized by mixing hesperetin and the Fe 3 O 4 nano-particles in phosphate buffer solution with the cross-linking agents of EDC and NHS. Fourier transform infrared spectroscopy proved that hesperetin is linked with the Fe 3 O 4 nano-particles. Thermal gravimetric analysis demonstrates that the weight ratio of hesperetin with respect to the Fe 3 O 4 /hesperetin compound is about 23.7%. The measured thermal changes of the Fe 3 O 4 nano-particles and the Fe 3 O 4 /hesperetin compound by radio frequency exposure for 60 minutes are 2.3 and 6.5°C, respectively.