Photon-counting photobleaching measurements and the effect of dispersion in two-photon microscopy

The effect of dispersion of the excitation pulse in two-photon microscopy is examined with a photon-counting microscope in both stationary and scanning modalities, and it is demonstrated that transform-limited pulses provide the best signal yield despite increased bleaching rates.

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Bibliographische Detailangaben
Hauptverfasser:	Field, J.J., Carriles, R., Squier, J.A.
Format:	Tagungsbericht
Sprache:	eng
Schlagworte:	(170.6280) Spectroscopy (180.2520) Fluorescence Microscopy Biomedical optical imaging Bleaching Dispersion Fluorescence fluorescence and luminescence Optical microscopy Optical pulse shaping Optical scattering Photobleaching Pulse measurements Pulse shaping methods
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Beschreibung
Zusammenfassung:	The effect of dispersion of the excitation pulse in two-photon microscopy is examined with a photon-counting microscope in both stationary and scanning modalities, and it is demonstrated that transform-limited pulses provide the best signal yield despite increased bleaching rates.
ISSN:	2160-9004
DOI:	10.1364/CLEO.2009.JTuD58