Backprojection-based reconstruction and correction for distance-dependent defocus in cryoelectron microscopy

We study two methods of micrograph processing in cryo- electron microscopy: the defocus-gradient corrected back- projection algorithm and the correction of micrographs for the contrast transfer function based on the frequency-distance relation. Analyzing integral images produced by the methods we co...

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Hauptverfasser:	Kazantsev, I.G., Herman, G.T., Cernetic, L.
Format:	Tagungsbericht
Sprache:	eng
Schlagworte:	Computed tomography Computer graphics Computer science CTF correction Electron microscopy Frequency Image analysis Image reconstruction Materials science and technology Transfer functions Transmission electron microscopy
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Beschreibung
Zusammenfassung:	We study two methods of micrograph processing in cryo- electron microscopy: the defocus-gradient corrected back- projection algorithm and the correction of micrographs for the contrast transfer function based on the frequency-distance relation. Analyzing integral images produced by the methods we conclude that they are asymptotically equivalent within the framework of stationary phase approximation.
ISSN:	1945-7928 1945-8452
DOI:	10.1109/ISBI.2008.4540950