Addressing cell-sourcing limitations with gene therapy

Genetic engineering with Run/spl times/2/Cbfa 1 for an alternative to biological grafts. Forced Runx2 expression via retroviral gene delivery promotes osteoblastic differentiation and mineralization in bone marrow stromal cells and stimulates conversion of myoblasts and dermal fibroblasts into a min...

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Veröffentlicht in:	IEEE engineering in medicine and biology magazine 2003-09, Vol.22 (5), p.65-70
Hauptverfasser:	Garcia, A.J., Guldberg, R.E., Byers, B.A., Gersbach, C.A., Phillips, J.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological information theory Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Bone tissue Bovine Cell Differentiation - genetics Cells (biology) Cells, Cultured Encoding Gene expression Gene therapy Genetic engineering Genetic Therapy - methods Genetic Vectors Male Mice Mice, Inbred BALB C Mineralization Myoblasts - cytology Myoblasts - physiology Neoplasm Proteins - genetics Neoplasm Proteins - metabolism Osteoblasts - cytology Osteoblasts - physiology Protein Engineering - methods Rats Rats, Wistar Recombinant Proteins - metabolism Stromal Cells - cytology Stromal Cells - metabolism Transcription Factors - genetics Transcription Factors - metabolism
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Zusammenfassung:	Genetic engineering with Run/spl times/2/Cbfa 1 for an alternative to biological grafts. Forced Runx2 expression via retroviral gene delivery promotes osteoblastic differentiation and mineralization in bone marrow stromal cells and stimulates conversion of myoblasts and dermal fibroblasts into a mineralizing osteoblastic phenotype. This genetic engineering strategy provides a promising approach to overcome cell-sourcing limitations in bone tissue engineering.
ISSN:	0739-5175 1937-4186
DOI:	10.1109/MEMB.2003.1256274