Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD

In this study, a reversed-phase, high pressure liquid chromatography (RP-HPLC) method was applied in the quantitative analysis of lycorine in the aerial parts and bulbs of Galanthus alpinu s Sosn. var. alpinus collected from Rize during the fowering and fruiting seasons. A simple method to extract lycorine from the plant specimens was performed, using pre-packed columns with diatomaceous earth (Extrelut[R]). The chromatographic separation was achieved employing an isocratic system with a mobile phase including trifuoroacetic acid-water-acetonitrile (0.01:95:5) measured at a flow rate of 1 mL/min using a diode array detector (DAD). The lycorine content in the bulbs during the fowering and fruiting seasons was detected as 0.01576 and 0.02351 %, respectively. Also, validation studies showed that the method was specific, accurate and precise. Keywords: Galanthus alpinus Sosn. var. alpinus, Amaryllidaceae, lycorine, HPLC-DAD