Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line

Background and Aims: It has been suggested that thyrotropin-releasing hormone (TRH) may have functions beyond its fundamental regulating function. Previous studies have demonstrated that TRH promotes wound healing. We aimed to perform an in vitro study in fibroblasts to assess the role of TRH in wounds that frequently occur in diabetes. Thus, we investigated the effects of TRH in wound healing both under normoglycemic and hyperglycemic-conditions. Methods: L929 mouse fibroblast cell line was used in the experiments. The cell viability was confirmed with XTT. Then the scratch migration assay was used for assessing the wound healing. TRH was added to both control and hyperglycemia groups at 100 nM after the scratch was created. The wound areas were measured after 24 and 48 hours after the scratch. Results: TRH and/or hyperglycemia did not affect the cellular activity after 48 hours. TRH reduced the wound areas (42.6%) compared to the control (52.2%) after 24 hours. In the hyperglycemia group the wound area was 64.3% and 61.0% of initial area at the 24(th) and 48(th) hours respectively. TRH incubation reduced these wound areas to 55.2% and 47.1% of initial areas. Conclusion: TRH treatment accelerated wound healing in hyperglycemia, which indicates the positive effects of TRH in wounds, may occur in diabetes. Keywords: Hyperglycemia, wound healing, scratch migration assay, fibroblast