Electrophoretic Studies of Biologically Important Mixed Metal – Ascorbic Acid –Nitrilotriacetate Complexes

Quantitative indication of a complex formation comes from the estimation of the stability or formation constants characterizing the equilibria corresponding to the successive addition of ligands. The binary equilibria of metal (II) / (III)-ascorbic acid and also mixed equilibria metal (II) / (III)-ascorbic acid-NTA have been studied using ionophoretic technique. The stability constants of metal-ascorbic acid binary complexes are found to be 10^sup 3.77^, 10^sup 2.47^, 10^sup 2.27^ and that of metal-ascorbic acid-NTA mixed complexes have been found to be 10^sup 6.05^, 10^sup 5.93^, 10^sup 5.75^, for Fe(III), Cu(II) and Co(II) complexes, respectively at 25 °C and ionic strength I^sub c^ = 0.1 mol dm^sup -3^ (HClO^sub 4^). [PUBLICATION ABSTRACT]