Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

L. P. Brion, W. Cammer, L. M. Satlin, C. Suarez, B. J. Zavilowitz and V. L. Schuster Albert Einstein College of Medicine, Department of Pediatrics, Medicine, Bronx, New York 10461, USA. Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.