Subtotal but not unilateral nephrectomy induces hyperplasia and protooncogene expression
F. Terzi, C. Ticozzi, M. Burtin, V. Motel, H. Beaufils, D. Laouari, B. M. Assael and C. Kleinknecht Institut National de la Sante et de la Recherche Medicale Unite 192, Hopital Necker, Enfants-Malades, Paris, France. It is generally accepted that renal compensatory growth after unilateral nephrectom...
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Veröffentlicht in: | American journal of physiology. Renal physiology 1995-05, Vol.268 (5), p.793-F801 |
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Zusammenfassung: | F. Terzi, C. Ticozzi, M. Burtin, V. Motel, H. Beaufils, D. Laouari, B. M. Assael and C. Kleinknecht
Institut National de la Sante et de la Recherche Medicale Unite 192, Hopital Necker, Enfants-Malades, Paris, France.
It is generally accepted that renal compensatory growth after unilateral
nephrectomy (Uni) is due to prominent hypertrophy with no involvement of
protooncogenes. Neither the balance between hypertrophy and hyperplasia nor
the expression of the early-growth-related genes has been studied after
subtotal nephrectomy (Nx). The occurrence of cystic tubular dilatations
after Nx may suggest an excessive cell proliferation in this model. We
measured DNA, RNA, and protein content, number of nuclei per tubular
section, as well as c-fos, c-jun, c-myc, c-H-ras, c-sis, and c-erb-B2
protooncogene expression in kidneys taken at time of surgery and 2, 7, and
14 days after sham operation (control rats), Uni, or Nx. After Uni,
hyperplasia was greater than expected (+79% for DNA at day 14) and was
associated with moderate hypertrophy (+11% for protein/DNA ratio). After
Nx, compensatory growth was due only to hyperplasia (+117% for DNA at day
14), with unchanged protein/DNA ratio (vs. Uni, P < 0.02). The greater
hyperplasia after Nx was confirmed by nuclei counting. The protooncogene
mRNA expression was constantly absent in control and Uni rats, whereas that
of c-fos and c-jun genes was detected in Nx rats at day 14 with a 2- to
12-fold increment. The c-fos and c-jun protein levels were also increased
at that time in Nx rats. This suggests the following: 1) the cellular
events following Uni and Nx are not the same, and 2) the late protooncogene
expression in Nx exclusively could favor a particular type of cell
proliferation possibly more related with cystic formation than with actual
compensatory growth. |
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ISSN: | 0363-6127 0002-9513 1931-857X 2161-1157 1522-1466 |
DOI: | 10.1152/ajprenal.1995.268.5.f793 |