Chronic administration of OB protein decreases food intake by selectively reducing meal size in male rats
1 Institute for Animal Sciences, Swiss Federal Institute of Technology, 8092 Zurich, Switzerland; 2 E. W. Bourne Laboratory, New York Hospital-Cornell Medical Center, White Plains, New York 10605; and 3 Department of Metabolic Diseases, Hoffmann-La Roche, Nutley, New Jersey 07110 The potent hypop...
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Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1998-07, Vol.275 (1), p.180-R185 |
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Zusammenfassung: | 1 Institute for Animal
Sciences, Swiss Federal Institute of Technology, 8092 Zurich,
Switzerland; 2 E. W. Bourne Laboratory,
New York Hospital-Cornell Medical Center, White Plains, New York
10605; and 3 Department of
Metabolic Diseases, Hoffmann-La Roche, Nutley, New Jersey
07110
The potent
hypophagic effect of OB protein (OB) is well established, but the
mechanism of this effect is largely unknown. We investigated the
effects of chronic administration of a novel modified recombinant human
OB (Mod-OB) with a prolonged half-life (>48 h) on ad
libitum food intake, spontaneous meal patterns, and body weight in 24 adult, male Sprague-Dawley rats (body weight at study onset: 292 g).
Single daily subcutaneous injections of Mod-OB (4 mg/kg daily) for 8 consecutive days significantly reduced ad libitum food intake compared
with vehicle injections from injection day 3 through postinjection day
3 . Mod-OB-injected rats ate between 4.5 and 7.1 g (or 13-20%) per day less than controls, with the reduction primarily occurring during the dark period. Body weight gain
was significantly decreased in response to Mod-OB from injection day 8 until postinjection day
4 , with a maximum difference of 24 g
on postinjection day
3 . The reduction of food intake by
Mod-OB was mainly due to a 21-34% decrease in nocturnal
spontaneous meal size. There was no significant effect of Mod-OB on
nocturnal meal frequency or duration. Mod-OB also did not reliably
affect the size, duration, or frequency of diurnal meals.
Mod-OB-injected rats displayed no compensatory hyperphagia after the
injection period. These results indicate that chronically administered
OB selectively affects the mechanisms controlling meal size in male rats.
leptin; adiposity; obesity; satiety; spontaneous
feeding |
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ISSN: | 0002-9513 0363-6119 2163-5773 1522-1490 |
DOI: | 10.1152/ajpregu.1998.275.1.R180 |