A comparison of the metabolic cost of protein synthesis in stenothermal and eurythermal isopod crustaceans
N. M. Whiteley, E. W. Taylor and A. J. el Haj School of Biological Sciences, University of Birmingham, United Kingdom. To examine the presence of metabolic cold adaptation in Antarctic isopods, whole animal rates of oxygen uptake (MVo2) and protein synthesis were measured in Glyptonotus antarcticus...
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Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1996-11, Vol.271 (5), p.1295-R1303 |
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Zusammenfassung: | N. M. Whiteley, E. W. Taylor and A. J. el Haj
School of Biological Sciences, University of Birmingham, United Kingdom.
To examine the presence of metabolic cold adaptation in Antarctic isopods,
whole animal rates of oxygen uptake (MVo2) and protein synthesis were
measured in Glyptonotus antarcticus at 0 degree C and compared with the
temperature isopod Idotea rescata at 4 and 14 degrees C. The specific
relationship between rates of metabolism and protein synthesis was
investigated by injecting animals with cycloheximide, a protein synthesis
inhibitor. In G. antarcticus, routine MVo2 was 11.10 +/- 0.89
mumol.kg-1.min-1 (n = 19 animals), and ks was 0.24 +/- 0.04% protein
synthesized/day (n = 8 animals). Comparison with I. rescata showed that
standardized whole animal MVo2 decreased with temperature (temperature
quotient = 1.99), but whole animal ks was considerably lower in the
Antarctic isopod; 66 and 22% of total MVo2 was attributable to protein
synthesis in G. antarcticus at 0 degree C and I. rescata at 4 degrees C,
respectively. The energetic cost of protein synthesis was four times higher
in G. antarcticus at 885 +/- 141 mmol ATP/g protein (n = 5 animals)
compared with 237 +/- 76 mmol ATP/g protein (n = 6) in I. rescata. G.
antarcticus does not show metabolic rate compensation and maintains
extremely low ks levels because of the relatively high energetic cost of
protein synthesis. |
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ISSN: | 0363-6119 0002-9513 1522-1490 |
DOI: | 10.1152/ajpregu.1996.271.5.r1295 |