Increased secretion of urokinase-type plasminogen activator by human lung microvascular endothelial cells
1 Fifth Department of Internal Medicine, Tokyo Medical College, Ibaraki 300-0395; and 2 Third Department of Internal Medicine and 3 Department of Anatomy, National Defense Medical College, Saitama 359-8513, Japan Human lung microvascular endothelial cells (HLMECs) secreted 1.5-15 times more uroki...
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Veröffentlicht in: | American journal of physiology. Lung cellular and molecular physiology 1998-07, Vol.275 (1), p.47-L54 |
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Zusammenfassung: | 1 Fifth Department of Internal Medicine, Tokyo
Medical College, Ibaraki 300-0395; and 2 Third
Department of Internal Medicine and 3 Department
of Anatomy, National Defense Medical College, Saitama 359-8513, Japan
Human lung microvascular endothelial cells
(HLMECs) secreted 1.5-15 times more urokinase-type plasminogen
activator (uPA) antigen than human hepatic microvascular endothelial
cells, human umbilical vein endothelial cells (HUVECs), angioma
endothelial cells, and lung fibroblasts. All of these cells also
secreted a 100-fold greater amount of plasminogen activator inhibitor-1 than of uPA antigen, and uPA activities were not detected in the culture medium. The expression of uPA mRNA in HLMECs was higher (100-fold) compared with HUVECs, angioma endothelial cells, and lung
fibroblasts. HLMECs secreted uPA antigen on both the luminal and basal
sides of the cells. On the other hand, HLMECs secreted a 10- to 15-fold
lower amount of tissue-type plasminogen activator than HUVECs, mostly
on the luminal side. After stimulation with interleukin (IL)-1 ,
HLMECs secreted a six- to ninefold amount of uPA antigen. In contrast,
no stimulatory effect was observed in HUVECs even under high IL-1
concentrations. The secretion of uPA and plasminogen activator
inhibitor-1 from HLMECs was also enhanced by tumor necrosis factor-
and IL-2. These results suggest that HLMECs may contribute not only to
the patency of lung vessels but also to the maintenance of alveolar
functions through the production and secretion of uPA, especially in
the presence of inflammatory cytokines.
tissue-type plasminogen activator; plasminogen activator
inhibitor-1; human umbilical vein endothelial cells |
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ISSN: | 1040-0605 0002-9513 1522-1504 |
DOI: | 10.1152/ajplung.1998.275.1.l47 |