Permeability: theory vs. practice in lung research
B. T. Peterson Department of Physiology, University of Texas Health Center, Tyler 75710. An increase in permeability of the pulmonary endothelium or epithelium to proteins and other macromolecules is an important part of many types of lung injury. Assessment of lung alveolar-capillary membrane integ...
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Veröffentlicht in: | American journal of physiology. Lung cellular and molecular physiology 1992-03, Vol.262 (3), p.243-L256 |
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Sprache: | eng |
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Zusammenfassung: | B. T. Peterson
Department of Physiology, University of Texas Health Center, Tyler 75710.
An increase in permeability of the pulmonary endothelium or epithelium to
proteins and other macromolecules is an important part of many types of
lung injury. Assessment of lung alveolar-capillary membrane integrity has
been the focus of many investigations, but no single method is best for all
conditions. Methods using in vitro preparations, such as cell monolayers,
provide the most accurate estimates of biophysical parameters defined from
membrane theory, but these models may not accurately represent the in vivo
condition. Methods using in vivo preparations, such as indicator dilution
techniques and the sheep lung-lymph preparation, are more accurate
representations of the clinical condition, but considerable assumptions
must be made to estimate the parameters of permeability. The most
productive studies of lung permeability make use of at least two methods
carefully selected according to their strengths and weaknesses. The
challenge is to select the right tools for the job and to interpret the
results in light of the weaknesses of the methods used. This will lead to a
better understanding of the role of altered permeability in the
pathophysiology of lung injury. |
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ISSN: | 1040-0605 0002-9513 1522-1504 |
DOI: | 10.1152/ajplung.1992.262.3.l243 |