Isolation, mapping, and regulated expression of the gene encoding mouse C-type natriuretic peptide
H. Huang, C. G. Acuff and M. E. Steinhelper Department of Medicine, University of Alabama at Birmingham 35294, USA. Genomic sequences encoding mouse C-type natriuretic peptide (CNP) were isolated from bacteriophage libraries and characterized by restriction enzyme and sequence analysis. The mouse CN...
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Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 1996-10, Vol.271 (4), p.H1565-H1575 |
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Zusammenfassung: | H. Huang, C. G. Acuff and M. E. Steinhelper
Department of Medicine, University of Alabama at Birmingham 35294, USA.
Genomic sequences encoding mouse C-type natriuretic peptide (CNP) were
isolated from bacteriophage libraries and characterized by restriction
enzyme and sequence analysis. The mouse CNP gene (Nppc) comprised at least
two exons and one intron and included several cis-regulatory elements in
the 5'-flanking sequence. The deduced amino acid sequence of mouse CNP-22
was identical to other mammalian CNPs. Analysis of allele distributions in
interspecific back-cross and recombinant inbred strains assigned Nppc to
chromosome 1. CNP transcripts were detected by ribonuclease protection
analysis in brain, ovary, and uterus, with lower levels in testes and
epididymus. Uterine CNP transcripts and protein were low in sexually
immature mice and adults at estrus and increased at proestrus, but similar
variations in ovarian CNP expression were not statistically significant.
Atrial natriuretic peptide and B-type natriuretic peptide transcripts were
not detected in mouse ovary or uterus. Thus CNP gene expression is
regulated by tissue-specific and inducible mechanisms in female
reproductive organs. Correlations between CNP expression and uterine fluid
content suggest that CNP may regulate uterine fluid balance in mice and
other mammals. |
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ISSN: | 0363-6135 0002-9513 1522-1539 |
DOI: | 10.1152/ajpheart.1996.271.4.h1565 |