Microinjection of vasopressin into the locus coeruleus of conscious rats
K. H. Berecek, H. R. Olpe, R. S. Jones and K. G. Hofbauer To determine whether vasopressin plays a role in central neural control of cardiovascular function by acting on the locus coeruleus we monitored arterial pressure and heart rate responses to graded injections of vasopressin (1-30 ng, 0.1-0.3...
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Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 1984-10, Vol.247 (4), p.H675-H681 |
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Zusammenfassung: | K. H. Berecek, H. R. Olpe, R. S. Jones and K. G. Hofbauer
To determine whether vasopressin plays a role in central neural control of
cardiovascular function by acting on the locus coeruleus we monitored
arterial pressure and heart rate responses to graded injections of
vasopressin (1-30 ng, 0.1-0.3 microliter) in the locus coeruleus of
conscious, restrained rats. Cannulas were stereotaxically implanted in the
locus coeruleus 2-5 days prior to experiment. Injections of vasopressin
into the locus coeruleus produced dose-related increases in mean arterial
pressure (12 +/- 2 to 57 +/- 6 mmHg) and heart rate (27 +/- 6 to 123 +/- 16
beats/min), which lasted over 1 h at the highest dose. Injection of the
antipressor vasopressin antagonist d(CH2)5Tyr(Me) arginine vasopressin (10
ng) into the locus coeruleus blocked the cardiovascular responses to
vasopressin. Administration of vasopressin into an area lateral to the
locus coeruleus had no effect on mean arterial pressure but produced an
increase in heart rate. Equivalent doses of saline, angiotensin II, and
norepinephrine (NE) had minimal or opposite (NE) effects on arterial
pressure and heart rate. Peripheral alpha-adrenergic blockade with
phentolamine and beta-adrenergic blockade with propranolol blocked the
cardiovascular responses to injection of vasopressin in the locus
coeruleus. These results suggest that vasopressin may act in the region of
the locus coeruleus to exert a central action on the cardiovascular system
that is mediated by a stimulation of sympathetic outflow. |
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ISSN: | 0363-6135 0002-9513 1522-1539 |
DOI: | 10.1152/ajpheart.1984.247.4.h675 |