Summation of behavioral and immunological stress: metabolic consequences to the growing mouse

Department of Animal Science, Stress Research Unit, University of California, Davis, California 95616 To address the hypothesis that multiple stressors can have cumulative effects on the individual, we determined the effects of restraint (R) stress (4 h/day for 7 days), immunological (L) stress [lipopolysaccharide (LPS) injection, 0.45 µg/g body wt on days 6 and 7 ], and R + L (RL) on the growth and energetics of C57Bl/6 male mice. R and L each repeatedly increased ( P 8 times), but RL caused even greater (>250%, P