Bacillus galactosidilyticus sp. nov., an alkali-tolerant {beta}-galactosidase producer

1 Department of Animal Product Quality, Center for Agricultural Research-Ghent, Brusselsesteenweg 370, B-9090 Melle, Belgium 2 School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK 3 Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium Correspondence Marc Heyndrickx M.Heyndrickx{at}clo.fgov.be A novel Bacillus isolate from raw milk and four strains from diverse origins that were identified previously as Bacillus lentus , Bacillus firmus and Bacillus circulans showed a high degree of similarity in amplified rDNA restriction analysis, SDS-PAGE and routine phenotypic tests, whilst 16S rDNA sequence comparisons and DNA relatedness data showed that this taxon was different from related Bacillus species. On the basis of these data, Bacillus galactosidilyticus sp. nov. is proposed, with the type strain LMG 17892 T (=DSM 15595 T =Logan B2188 T =MB 800 T ). Abbreviations: ARDRA, amplified rDNA restriction analysis Published online ahead of print on 31 October 2003 as DOI 10.1099/ijs.0.02816-0. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Bacillus galactosidilyticus LMG 17892 T and Bacillus sp. R-6760, both determined in this work, are AJ535638 and AJ535639 , respectively. Supplementary figures showing a full phylogenetic tree and normalized computer profiles from SDS-PAGE analysis are available as supplementary material in IJSEM Online.