Nuclear Factor-κB Activity Correlates with Growth, Angiogenesis, and Metastasis of Human Melanoma Cells in Nude Mice
The purpose of this study was to determine the role of nuclear factor (NF)-κB/relA activity in the induction of angiogenesis and production of metastasis by human melanoma cells. Highly metastatic melanoma variant cells expressed high levels of constitutive NF-κB activity. Transfection of highly m...
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Veröffentlicht in: | Clinical cancer research 2000-06, Vol.6 (6), p.2573 |
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Sprache: | eng |
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Zusammenfassung: | The
purpose of this study was to determine the role of nuclear factor
(NF)-κB/relA activity in the induction of angiogenesis and production
of metastasis by human melanoma cells. Highly metastatic melanoma
variant cells expressed high levels of constitutive NF-κB activity.
Transfection of highly metastatic human melanoma variant cells with a
dominant-negative mutant inhibitor of nuclear factor-κBα
(Iκβα) expression vector (IκβαM) decreased the level of
constitutive NF-κB activity, inhibited s.c. tumor growth, and
prevented lung metastasis in nude mice. Furthermore, the slow-growing
s.c. tumors formed by the IκBαM-transfected cells exhibited a
decrease in microvessel density (angiogenesis), which correlated with a
decrease in the level of interleukin-8 expression. Collectively, these
results demonstrate that NF-κB/relA activity significantly
contributes to tumorigenicity, angiogenesis, and metastasis of human
melanoma cells implanted in nude mice. |
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ISSN: | 1078-0432 1557-3265 |