Guide Molecule-driven Stereospecific Degradation of α-Methylpolyamines by Polyamine Oxidase

FAD-dependent polyamine oxidase (PAO; EC 1.5.3.11) is one of the key enzymes in the catabolism of polyamines spermidine and spermine. The natural substrates for the enzyme are N 1 -acetylspermidine, N 1 -acetylspermine, and N 1 , N 12 -diacetylspermine. Here we report that PAO, which normally metabolizes achiral substrates, oxidized ( R )-isomer of 1-amino-8-acetamido-5-azanonane and N 1 -acetylspermidine as efficiently while ( S )-1-amino-8-acetamido-5-azanonane was a much less preferred substrate. It has been shown that in the presence of certain aldehydes, the substrate specificity of PAO and the kinetics of the reaction are changed to favor spermine and spermidine as substrates. Therefore, we examined the effect of several aldehydes on the ability of PAO to oxidize different enantiomers of α-methylated polyamines. PAO supplemented with benzaldehyde predominantly catalyzed the cleavage of ( R )-isomer of α-methylspermidine, whereas in the presence of pyridoxal the ( S )-α-methylspermidine was preferred. PAO displayed the same stereospecificity with both singly and doubly α-methylated spermine derivatives when supplemented with the same aldehydes. Structurally related ketones proved to be ineffective. This is the first time that the stereospecificity of FAD-dependent oxidase has been successfully regulated by changing the supplementary aldehyde. These findings might facilitate the chemical regulation of stereospecificity of the enzymes.