Exploratory and Confirmatory Gene Expression Profiling of mac1Î
Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated...
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Veröffentlicht in: | The Journal of biological chemistry 2004-02, Vol.279 (6), p.4450 |
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Hauptverfasser: | , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in
Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif
between up- and down-regulated genes, and we identified new genes potentially regulated by Aft1p/Aft2p. In addition, multiple
genes encoding proteins containing Fe-S clusters were down-regulated suggesting metabolic reorganization to conserve iron
in mac1 Î. Null mutants of each of the differentially expressed genes were characterized for copper- or iron-related phenotypes. New
or additional support for a role in copper and iron homeostasis is provided in this study for the gene products of AKR1, MRS4, PCA1, SSU1, TIS11, YBR047W, YHL035C, YHR045W, YLR047C, YLR126C , and YTP1 . |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M212308200 |