The CCAAT Enhancer-binding Protein (C/EBP)β and Nrf1 Interact to Regulate Dentin Sialophosphoprotein (DSPP) Gene Expression during Odontoblast Differentiation
Terminal differentiation of odontoblasts, the principal cells in dentin formation, proceeds by synthesis of type I collagen and noncollagenous proteins. DSP and DPP are specific markers for terminally differentiated odontoblasts and are encoded by a single gene DSPP (dentin sialophosphoprotein). In...
Gespeichert in:
Veröffentlicht in: | The Journal of biological chemistry 2004-10, Vol.279 (44), p.45423 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Terminal differentiation of odontoblasts, the principal cells in dentin formation, proceeds by synthesis of type I collagen
and noncollagenous proteins. DSP and DPP are specific markers for terminally differentiated odontoblasts and are encoded by
a single gene DSPP (dentin sialophosphoprotein). In an attempt to understand the molecular mechanisms required for tissue-specific expression
of the DSPP gene, we have identified a novel interaction between two bZIP transcription factors, Nrf1 and the CCAAT enhancer-binding
protein (C/EBP)β. This interaction was confirmed by both immunoprecipitation and chromatin immunoprecipitation assays. In
undifferentiated odontoblasts, Nrf1 and C/EBPβ repress DSPP promoter activity individually and synergistically by cooperatively interacting with each other. This mutual interaction
is facilitated by the bZIP domains in both the proteins. The repression domain in both Nrf1 and C/EBPβ was determined, and
deletion of this domain abolished transcriptional repression. In fully differentiated odontoblasts, the loss of interaction
between Nrf1 and C/EBPβ results in an increased DSPP transcription. Further, this interaction was found to be dependent on
phosphorylation at Ser 599 of Nrf1. Thus, the physical interaction between Nrf1 and C/EBPβ provide a novel mechanism for the transcriptional regulation
of DSPP in odontoblasts. |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M405031200 |