Promoter Sequences in the RI Subunit Gene of cAMP-dependent Protein Kinase Required for Transgene Expression in Mouse Brain

Neural-specific expression of the mouse regulatory type-Iβ (RIβ) subunit gene of cAMP-dependent protein kinase is controlled by a fragment of genomic DNA comprised of a TATA-less promoter flanked by 1.5 kilobases of 5′-upstream sequence and a 1.8-kilobase intron. This DNA contains a complex arrangement of transcription factor binding motifs, and previous experiments have shown that many of these are recognized by proteins found in brain nuclear extract. To identify sequences critical for RIβ expression in functional neurons, we performed a deletion analysis in transgenic mice. Evidence is presented that the GC-rich proximal promoter is responsible for cell type-specific expression in vivo because RIβ DNA containing as little as 17 base pairs (bp) of 5′-upstream sequence was functional in mouse brain. One likely regulatory element coincides with the start of transcription and includes an EGR-1 motif and 3 consecutive SP1 sites within a 21-bp interval. Maximal RIβ promoter activity required the adjacent 663 bp of 5′-upstream DNA where most, but not all, of the regulatory activity was localized between position −663 and −333. A 37-bp direct repeat lies within this region that contains 2 basic helix-loop-helix binding sites, each of which are overlapped by two steroid hormone receptor half-sites, and a shared AP1 consensus sequence. Intron I sequences were also tested, and deletion of a 388-bp region containing numerous Sp1-like sequences lowered transgene activity significantly. These results have identified specific regions of the RIβ promoter that are required for the expression of this signal transduction protein in mouse neurons.