Molecular Basis of the Soluble and Membrane-bound Forms of the Murine Leukemia Inhibitory Factor Receptor -Chain
The murine leukemia inhibitory factor receptor α-chain (mLIFR) exists in a membrane-bound and a soluble form. The two major classes of mRNA transcript correspond to either the soluble or membrane-bound form of the mLIFR. In this study we have identified a complex and heterogeneous pattern of expres...
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Veröffentlicht in: | The Journal of biological chemistry 1996-03, Vol.271 (10), p.5495 |
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Sprache: | eng |
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Zusammenfassung: | The murine leukemia inhibitory factor receptor α-chain (mLIFR) exists in a membrane-bound and a soluble form. The two major
classes of mRNA transcript correspond to either the soluble or membrane-bound form of the mLIFR. In this study we have identified
a complex and heterogeneous pattern of expression of mRNA transcripts for this receptor in normal mouse tissues and cell lines.
In order to understand the molecular basis of these transcripts, genomic clones encompassing the region of divergence from
the soluble to the membrane-bound form of the receptor were isolated. cDNAs encoding the membrane-bound form of the mLIFR
were generated by an alternative splicing event where an exon that is specific to the soluble mLIFR was skipped. The membrane-bound
form of the mLIFR was heterogeneously polyadenylated with at least five different sites of polyadenylation. The mRNA transcript
encoding the soluble form of the mLIFR contained a region highly homologous to a murine B2 repetitive element, thus providing
a possible explanation for the genesis of this transcript. The different forms of the mLIFR were analyzed in a wide range
of mouse tissues in pseudopregnant mice and in mice at various stages of pregnancy. Only liver, placenta, and uterus showed
an increase in the levels of mLIFR mRNA expression during pregnancy, indicating an important role for the LIFR in this process.
However, somewhat surprisingly, there was no detectable difference in mLIFR mRNA levels or levels of soluble protein in leukemia
inhibitory factor nullizygous mice when compared with normal mice. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.271.10.5495 |