False-Negative PCR Result Due to Gene Polymorphism: the Example of Neisseria meningitidis

Early treatment of meningococcal meningitis is mandatory but may negate the cerebrospinal fluid culture. Etiological diagnosis then mainly relies on PCR. Here, we report a case of false-negative results for real-time PCR for a Neisseria meningitidis serogroup B isolate with a polymorphism in the ctr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of Clinical Microbiology 2010-12, Vol.48 (12), p.4590-4591
Hauptverfasser:	Jaton, Katia, Ninet, Béatrice, Bille, Jacques, Greub, Gilbert
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacteriological Techniques - methods Bacteriology Biological and medical sciences Child, Preschool DNA, Bacterial - chemistry DNA, Bacterial - genetics False Negative Reactions Fundamental and applied biological sciences. Psychology Humans Male Meningococcal Infections - diagnosis Meningococcal Infections - microbiology Microbiology Miscellaneous Molecular Sequence Data Neisseria meningitidis Neisseria meningitidis - genetics Neisseria meningitidis - isolation & purification Polymerase Chain Reaction - methods Polymorphism, Genetic Sequence Analysis, DNA
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Early treatment of meningococcal meningitis is mandatory but may negate the cerebrospinal fluid culture. Etiological diagnosis then mainly relies on PCR. Here, we report a case of false-negative results for real-time PCR for a Neisseria meningitidis serogroup B isolate with a polymorphism in the ctrA gene.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.01766-10