Novel Use of Tryptose Sulfite Cycloserine Egg Yolk Agar for Isolation of Clostridium perfringens during an Outbreak of Necrotizing Enterocolitis in a Neonatal Unit

Clostridium perfringens has been associated with necrotizing enterocolitis (NEC), which is a serious disease of neonates. Our study describes the novel use of selective tryptose sulfite cycloserine with egg yolk agar (TSC-EYA) during a nursery outbreak. This medium provides a rapid, sensitive, and a...

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Veröffentlicht in:	Journal of Clinical Microbiology 2010-11, Vol.48 (11), p.4263-4265
Hauptverfasser:	Kotsanas, Despina, Carson, Jolene A, Awad, Milena M, Lyras, Dena, Rood, Julian I, Jenkin, Grant A, Stuart, Rhonda L, Korman, Tony M
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar Bacteriological Techniques - methods Bacteriology Biological and medical sciences Clostridium Infections - diagnosis Clostridium Infections - epidemiology Clostridium Infections - microbiology Clostridium perfringens Clostridium perfringens - isolation & purification Culture Media - chemistry Cycloserine - metabolism Disease Outbreaks Egg Yolk - metabolism Enterocolitis, Necrotizing - epidemiology Enterocolitis, Necrotizing - microbiology Fundamental and applied biological sciences. Psychology Humans Infant, Newborn Microbiology Organic Chemicals - metabolism Sensitivity and Specificity Sulfites - metabolism
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Zusammenfassung:	Clostridium perfringens has been associated with necrotizing enterocolitis (NEC), which is a serious disease of neonates. Our study describes the novel use of selective tryptose sulfite cycloserine with egg yolk agar (TSC-EYA) during a nursery outbreak. This medium provides a rapid, sensitive, and accurate presumptive identification of C. perfringens.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.01724-10