Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene

Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PC...

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Veröffentlicht in:	Journal of Clinical Microbiology 1995-05, Vol.33 (5), p.1344-1347
Hauptverfasser:	Battles, J.K. (Program Resources, Inc./DynCorp.), Williamson, J.C, Pike, K.M, Gorelick, P.L, Ward, J.M, Gonda, M.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals ARN RIBOSOMAL ARN RIBOSOMIAL BACTERIA GRAM NEGATIVA BACTERIE GRAM NEGATIF Bacteriological Techniques - statistics & numerical data Base Sequence Conserved Sequence DIAGNOSTIC DIAGNOSTICO DNA Primers - genetics DNA, Bacterial - genetics DNA, Ribosomal - genetics GENE GENES Genes, Bacterial Helicobacter - genetics Helicobacter - isolation & purification Helicobacter hepaticus Helicobacter Infections - diagnosis Helicobacter Infections - microbiology Humans Male Mice Mice, SCID Molecular Sequence Data Nucleic Acid Hybridization PCR Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data RATON RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics SECUENCIA NUCLEOTIDICA Sensitivity and Specificity SEQUENCE NUCLEOTIDIQUE SOURIS
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Zusammenfassung:	Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequences
ISSN:	0095-1137 1098-660X
DOI:	10.1128/jcm.33.5.1344-1347.1995