Transformation Frequency of a mariner-Based Transposon in Rickettsia rickettsii

Transformation frequencies of a mariner-based transposon system in Rickettsia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants. Sequence analysis of insertion sites in both R. rickettsii and R. prowazekii indicated that insertions were random. Transposo...

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Veröffentlicht in:	Journal of Bacteriology 2011-09, Vol.193 (18), p.4993-4995
Hauptverfasser:	Clark, Tina R, Lackey, Amanda M, Kleba, Betsy, Driskell, Lonnie O, Lutter, Erika I, Martens, Craig, Wood, David O, Hackstadt, Ted
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Biological and medical sciences DNA Transposable Elements - genetics DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology genetic transformation Gram-negative bacteria Microbiology Miscellaneous Molecular Biology of Pathogens Mutagenesis Mutagenesis, Insertional mutants Mutation Rickettsia prowazekii - genetics Rickettsia rickettsii Rickettsia rickettsii - genetics sequence analysis Transformation, Genetic transposons Viral Plaque Assay
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Zusammenfassung:	Transformation frequencies of a mariner-based transposon system in Rickettsia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants. Sequence analysis of insertion sites in both R. rickettsii and R. prowazekii indicated that insertions were random. Transposon mutagenesis provides a useful tool for rickettsial research.
ISSN:	0021-9193 1098-5530 1067-8832
DOI:	10.1128/JB.05279-11