Biochemical and Mutational Studies of the Bacillus cereus CECT 5050T Formamidase Support the Existence of a C-E-E-K Tetrad in Several Members of the Nitrilase Superfamily

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Veröffentlicht in:	Applied and Environmental Microbiology 2011-08, Vol.77 (16), p.5761-5769
Hauptverfasser:	Pablo Soriano-Maldonado, Ana Isabel Martínez-Gómez, Montserrat Andújar-Sánchez, José L. Neira, Josefa María Clemente-Jiménez, Francisco Javier Las Heras-Vázquez, Felipe Rodríguez-Vico, Sergio Martínez-Rodríguez
Format:	Artikel
Sprache:	eng
Schlagworte:	Amidohydrolases - chemistry Amidohydrolases - genetics Aminohydrolases - metabolism Ammonia Bacillus cereus - enzymology Bacillus cereus - genetics Bacteria Bacterial Proteins - chemistry Bacterial Proteins - genetics Base Sequence Biological and medical sciences Catalysis Chromatography, Gel Chromatography, High Pressure Liquid Circular Dichroism - methods Cloning, Molecular Enzyme Activation Enzyme Assays Enzymology and Protein Engineering Escherichia coli - chemistry Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Glutamic Acid - chemistry Microbiology Molecular Sequence Data Mutagenesis, Site-Directed Mutation Pathogenesis Phylogeny Protein Structure, Secondary Proteins Recombinant Proteins - chemistry Sequence Alignment Sequence Homology, Amino Acid Substrate Specificity
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Zusammenfassung:	Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: AEM
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.00312-11