L-Ribose Production from L-Arabinose by Using Purified L-Arabinose Isomerase and Mannose-6-Phosphate Isomerase from Geobacillus thermodenitrificans

Two enzymes, L-arabinose isomerase and mannose-6-phosphate isomerase, from Geobacillus thermodenitrificans produced 118 g/liter L-ribose from 500 g/liter L-arabinose at pH 7.0, 70°C, and 1 mM Co²⁺ for 3 h, with a conversion yield of 23.6% and a volumetric productivity of 39.3 g liter⁻¹ h⁻¹....

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Veröffentlicht in:	Applied and Environmental Microbiology 2009-11, Vol.75 (21), p.6941-6943
Hauptverfasser:	Yeom, Soo-Jin, Kim, Nam-Hee, Park, Chang-Su, Oh, Deok-Kun
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldose-Ketose Isomerases - isolation & purification Aldose-Ketose Isomerases - metabolism Arabinose - metabolism Bacteria Biotechnology Cobalt - pharmacology Enzyme Activators - pharmacology Enzymes Geobacillus Geobacillus - enzymology Hydrogen-Ion Concentration Mannose-6-Phosphate Isomerase - isolation & purification Mannose-6-Phosphate Isomerase - metabolism Ribose - metabolism Temperature Time Factors
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Beschreibung
Zusammenfassung:	Two enzymes, L-arabinose isomerase and mannose-6-phosphate isomerase, from Geobacillus thermodenitrificans produced 118 g/liter L-ribose from 500 g/liter L-arabinose at pH 7.0, 70°C, and 1 mM Co²⁺ for 3 h, with a conversion yield of 23.6% and a volumetric productivity of 39.3 g liter⁻¹ h⁻¹.
ISSN:	0099-2240 1098-5336 1098-6596
DOI:	10.1128/AEM.01867-09