Flavobacterium sp. Strain 4221 and Pedobacter sp. Strain 4236 β-1,3-Glucanases That Are Active at Low Temperatures

Flavobacterium sp. Strain 4221 and Pedobacter sp. Strain 4236 β-1,3-Glucanases That Are Active at Low Temperatures

Secretion of β-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of β-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hyd...

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Veröffentlicht in:Applied and Environmental Microbiology 2008-11, Vol.74 (22), p.7070-7072
Hauptverfasser: Rasmussen, Mikkel A, Madsen, Søren M, Stougaard, Peter, Johnsen, Mads G
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Bacteroidetes - enzymology
Base Sequence
Biological and medical sciences
Cell Wall - metabolism
Cloning, Molecular
Cold Temperature
Enzymology and Protein Engineering
Escherichia coli - genetics
Flavobacterium - enzymology
Fundamental and applied biological sciences. Psychology
Gene Expression
Glucan 1,3-beta-Glucosidase - metabolism
Lactococcus lactis - genetics
Microbiology
Molecular Sequence Data
Temperature
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Zusammenfassung:Secretion of β-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of β-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.00681-08