Analysis of Vaginal Lactobacilli from Healthy and Infected Brazilian Women

Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial va...

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Veröffentlicht in:	Applied and Environmental Microbiology 2008-07, Vol.74 (14), p.4539-4542
Hauptverfasser:	Martinez, Rafael C.R, Franceschini, Sílvio A, Patta, Maristela C, Quintana, Silvana M, Nunes, Álvaro C, Moreira, João L.S, Anukam, Kingsley C, Reid, Gregor, De Martinis, Elaine C.P
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of Variance Bacteria Biological and medical sciences Brazil - epidemiology Candidiasis Candidiasis, Vulvovaginal - microbiology Cell culture Chi-Square Distribution DNA, Bacterial - isolation & purification DNA, Ribosomal Spacer - isolation & purification Electrophoresis, Gel, Pulsed-Field Female Fundamental and applied biological sciences. Psychology Humans Hydrogen Peroxide - metabolism Infections Lactobacillus Lactobacillus - classification Lactobacillus - isolation & purification Lactobacillus - metabolism Microbiology Polymerase Chain Reaction Public Health Microbiology Reproductive system Ribonucleic acid RNA Vagina - microbiology Vaginal Discharge - microbiology Vaginosis, Bacterial - epidemiology Vaginosis, Bacterial - microbiology Women
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Beschreibung
Zusammenfassung:	Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H₂O₂-producing lactobacilli were not associated with protection against VVC.
ISSN:	0099-2240 1098-5336 1098-6596
DOI:	10.1128/AEM.00284-08