Evidence for Horizontal Transfer of 1-Aminocyclopropane-1-Carboxylate Deaminase Genes

PCR was used to rapidly identify and isolate 1-aminocyclopropane-1-carboxylate (ACC) deaminase genes from bacteria. The Shimodaira-Hasegawa test was used to assess whether phylogenetically anomalous gene placements suggestive of horizontal gene transfer (HGT) were significantly favored over vertical...

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Veröffentlicht in:	Applied and Environmental Microbiology 2005-11, Vol.71 (11), p.7556-7558
Hauptverfasser:	Hontzeas, N, Richardson, A. O, Belimov, A, Safronova, V, Abu-Omar, M. M, Glick, B. R
Format:	Artikel
Sprache:	eng
Schlagworte:	1-aminocyclopropane-1-carboxylate deaminase amidine hydrolases Bacteria Carbon-Carbon Lyases - genetics conjugation DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Ribosomal - analysis enzyme activity Gene Transfer, Horizontal Genes Gram-Negative Bacteria - enzymology Gram-Negative Bacteria - genetics horizontal gene transfer Molecular Sequence Data nucleotide sequences Phylogeny plant growth-promoting rhizobacteria Plant Microbiology Polymerase Chain Reaction Rhodococcus - enzymology Rhodococcus - genetics ribosomal RNA RNA, Ribosomal, 16S - genetics soil bacteria
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Zusammenfassung:	PCR was used to rapidly identify and isolate 1-aminocyclopropane-1-carboxylate (ACC) deaminase genes from bacteria. The Shimodaira-Hasegawa test was used to assess whether phylogenetically anomalous gene placements suggestive of horizontal gene transfer (HGT) were significantly favored over vertical transmission. The best maximum likelihood (ML) ACC deaminase tree was significantly more likely than four alternative ML trees, suggesting HGT.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.71.11.7556-7558.2005